FISH testing is recommended to support or confirm chromosome aberrations or when inconclusive results are found. If abnormalities are present, they are explained in a paragraph which helps to clarify and correlate clinical findings with pathological morphology. The final report identifies the chromosomal sex and modal chromosome number. The morphologic interpretation and correlation of results on all cases is performed by a board-certified doctoral level scientist (laboratory director). Both technologists analyze the first 10 consecutive metaphases after which they are reviewed by the manager and reported by the director. Analysis and karyotyping is carried out by two certified technologists using the Leica automated metaphase scanner and Cytovision software. They are harvested at 24 hours and if volume is sufficient, a 48 hour culture is also initiated followed by G-banding. Chromosome analysis on bone marrow serves as a diagnostic study for multiple relevant acquired chromosomal aberrations in one test, whereas molecular studies are significant in cases where a diagnosis has been made or a suspicion exists and specific testing is targeted.īone marrow samples are unstimulated. Thus, the study of chromosomal abnormalities in leukemia serves most importantly to assist in more accurate diagnosis and thereby providing prognostic information and allowing the more rational selection of therapy for patients. These consistent chromosomal aberrations acquired new significance with the localization of cellular transforming genes (oncogenes) to chromosome breakpoints that are involved in specific rearrangements. One example of these abnormalities is the 15 17 translocation which is identified only in patients with promyelocytic leukemia. In the hematologic malignant diseases, the recognized recurring chromosomal abnormalities often correlate with particular subtypes of leukemia that have characteristic morphologic and clinical features, such as response to therapy. It may also be relevant in cases to confirm or exclude the diagnosis of hematological malignancies based on morphology and track progress of treatment. Transportation: Avoid freezing or heating over 35 OCĪ chromosome analysis on bone marrow may be clinically significant to identify and diagnose acquired neoplastic conditions associated with indications such as, cytopenias, T-cell lymphoma, B-cell lymphoma, multiple myeloma, acute myeloid leukemia, acute lymphocytic lymphoma, chronic myeloid leukemia, chronic lymphocytic leukemia, myeloprolypherative disorder and myelodysplastic syndrome.

Stability at Room Temperature: 8 hours, then refrigerate Container: Sodium Heparin (green top tube)

Cytovision software manual